Growth, Survival and Haematological Responses of Heterobranchus bidorsalis Juveniles to Changes in Photoperiod

Friday Elijah Osho


The effects of artificial photoperiods on growth and haematology of Heterobranchus bidorsalis juveniles were assessed. Hetrobranchus bidorsalis (5.69±0.41g/100fish/m2) were randomly exposed to tanks representing different artificial photoperiods of 24L:0D (PD1), 18L:6D (PD2),
12L:12D (PD3), 6L:18D (PD4) and 0L: 24D (PD5) for 84 days. Feed intake, Mean weight gain (MWG), Feed Conversion Ratio (FCR), Specific Growth Rate (SGR) and Survival Rate were determined. Blood samples were analyzed for Packed cell volume (PCV), Red blood cells (RBC), Haemoglobin (Hb), White blood cells (WBC), platelets and white blood cell differentials. Data were subjected to one-way ANOVA and Fisher's LSD follow-up test was used to separate the means. Differences were considered significant at p <0.05 and correlation analysis was used to test relationship between variables. Feed intake showed no significant difference (p<0.05) in treatments, but was marginally higher in PD5. However, MWG was highest in PD4 and PD5 groups (22.30+ 4.48g and 20.40+5.63g respectively) while it was least in PD1 and PD2 (16.5+0.39sg and 15.0+3.77g). The FCR ranged between 1.2.±0.06 and 1.6±0.72, with the least values obtained in PD4 and PD5 while the highest value was in PD2. The PCV, RBC and WBC
ranged from 20.00±200 to 29.00±1.00, 1.30±0.14 to 2.10±0.22b

and 15,000±200 and
27166.70±202 respectively. PD1 and PD2 had higher lymphocyte: neutrophil ratio, 68:24 and
67:27, respectively compared to the control (PD3) which recorded 71:21. The findings indicate that exposure of H. bidorsalis juvenile to continuous light for long duration could affect feed consumption and growth performance as well as haematological parameters while extended period of darkness achieves better performance.

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Abdullah A.A. and Elsayed. M. Y. (1998). Effect of three photoperiods on the growth of tilapia fish Oreochromos aureus reared in glass tanks. Saudi. Journal of Biological Sciences Vol. 5,No

, pp 93-99.

Björnsson B.T, Hemre G.I., Bjørnevik M. and Hansen T. (2000). Photoperiod regulation of plasma growth hormone levels during induced smoltification of under yearling Atlantic salmon. Gen Comp Endocrinology 119, 17-25.

Bonnet E., Montfort J., Esquerre D., Hugot K, Fostier A and Bobe J. (2007). Effect of photoperiod manipulation on rainbow trout (Oncorhynchus mykiss) egg quality: a genomic study.Aquaculture 268, 13-22.

Boyd, C.E. (1979). Water quality in warm water fish ponds. Craftmaster Auburn, Alabama, USA, Printers Inc.

Bromage, N., Porter, M. and C. Randall C. (2001).

The environmental regulation of International centre for aquaculture. Agri Expt. Station, Auburn Univ. pp1-20.

Hart P.R., Hutchinson W.G. and Purser G.J. (1996).

Effects of photoperiod, temperature and salinity on hatchery reared larvae of the greenback flounder (Rhombosolea tapirina Gunther, 1862). Aquaculture 144, 303-311.

Helfrich-Förster C. (2003). The neuroarchitecture of the circadian clock in the brain of Drosophila melanogaster. Microsc Res Tech. 62 (2):94-102. Shan X. J, Quan H. F and Dou S. Z. 2008. Effects of delayed first feeding on growth and survival of rock bream Oplegnathus fasciatus larvae. Aquaculture 277, 14-23.

Jauro, I. A. and Usman, I. (2015). The Effect of Photoperiod on the Growth of African Catfish, (Clarias gariepinus Burchell, 1822) Juveniles in the Semi-Arid zone of Nigeria Nigerian Journal of Fisheries andAquaculture 3(1&2): 68 – 77,

Jobling M. and B. M. Baardvik (1994). The influence of environmental manipulations on inter– and intra–individual variation in food acquisition and growth performance of Arctic charr, Salvelinus alpi. Journal of fish biology Volume

, Issue 6 pp 1069–1087.

Koedprang W., Nakajima M., Maita M., Taniguchi N. (2002). Correlation of hematology and plasma chemistry levels in silver crucian carp Carassius langsdorfii. Fish Sci 68:721–728.

Lambert Y. and Dutil J. (2001). Food intake and growth of adult Atlantic cod (Gadus morhua L.) reared under different conditions of stocking density, feeding frequency and size-grading. Aquaculture 192, 233-247.

Moshood K. Mustapha, Benedict U. Okafor, Khalid S. Olaoti, Opeyemi K. Oyelakin (2012). Effects of three different photoperiods on the growth and body coloration of juvenile African catfish, Clarias gariepinus. Arch. Pol. Fish. 20: 55-59.

Odunze, F.C., Ibiwoye T.I.I., Ladu, B.M.B. and P.A Iyolyoon. (2009). Preliminary studies on the effects of light duration on the growth and performance of Clarias gariepinus fingerlings. National Institute for Freshwater Fisheries Research, P.M.B. 6006, New Bussa, Niger State, pp.189-194.

Omitoyin, B.O. (2007). Introduction to fish farming in Nigeria. University of Ibadan press. 90pp.

Petit G., Beauchaud M., Attia J. and Buisson B. (2003). Food intake and growth of largemouth bass (Micropterus salmoides) held under alternated light/dark cycle (12L: 12D) or exposed to continuous light. Aquaculture 228,


Ruchin A. B. (2007). Effect of photoperiod on growth, physiological and haematological indices of juvenile Siberian sturgeon Asipenser baerii. Biology Bulletin Volume 34,N0 6, pp 583-589.

Schreck, C.B. (1982). Stress and rearing of salmonids. Aquaculture 28: 241-9

Shahkar E, KimD,MohseniM,KharaH,YunHandBai S. C. (2015). Effects of Photoperiod Manipulation on Growth Performance and Hematological Responses of Juvenile Caspian Roach Rutilus rutilus caspicus Fish Aquat Sci .18(1), 51-56.

Simensen L.M., Jonassen T.M., Imsland A.K. and Stefansson S.O. (2000). Photoperiod regulation of growth of juvenile Atlantic halibut (Hip- poglossus hoppoglossus L.). Aquaculture 190,


Stoskopf, M.K. (1993). Clinical pathology in Stoskopf, M.K. (ed.) Fish Medicine Saunders, Philadelphia, pp113-131.

Strivastava S. (2003). Influence of continous light and darkness on the secretory pinealocytes of Heteropneustus fossilis; J. Biosci. 28(5), 613-

Taranger, G.L., Haux, C., Stefansson, S.O., Björnsson, B.T., Walther, B.T., Hansen, T., (1998). Abrupt changes in photoperiod affect age at maturity, timing of ovulation and plasma testosterone and

oestradiol-17â profiles in Atlantic salmon,

Salmo salar.Aquacult. 162, 85–98.

Thomas, P. and Robertson, L. (1991). Plasma contisol and glucose stress responses of red drum (Sciaenopaocellatos) to handling and shallow water stressor and anesthesia with MS22, quinaldine sulfate. Aquaculture. 80:210-220.

Wendelaar B., S.E. (1997). The stress response in fish. Physiological Reviews 77(3):591- 625.

Yanik T. and M.S. Aras, (1998). Effects of replacing slaughterhouse by-product meals in salmonid, Oncorhynchus mykiss, diets on body composition. pp. 549-557. In: Dogu Anadolu Bölgesi III. Su Ürünleri Sempozyumu, 10-12 Haziran 1998. Erzurum, Turkey.