Antibacterial Potentials of Selected Synthetic Antibiotics and Ocimum gratissimum against Some Bacteria of the Skin and Stomach of Clarias garienpinus Fingerlings

Azeez Ayodele Alimi, Adekunle Adedoyin Idowu, Oloruntobiloba Akinyemi

Abstract


The aim of this study was to investigate the antibacterial activity of some selected antibiotics and Ocimum gratissimum aqueous leaf extract against some bacteria isolated from Clarias garienpinus fingerlings and water samples obtained from a fish farm at Ijebu-Ode. Escherichia coli, Staphylococcus saprophyticus, Streptococcus spp., Aeromonas hydrophilia, Pseudomonas aeruginosa, Bacillus mycoides, Pseudomonas fluoresces, Proteus vulgaris, Klebsiella oxytoca and Micrococcus spp. were isolated from the skin and in the stomach of fish samples using standard microbiological methods. Sensitivity of the isolated bacteria to antibiotics was also determined. The mean Total Bacteria Count ( X 106CFU/mL) of skin was 2.46±1.31, stomach was 2.16±2.29 and that of water was 1.45±0.21. Result of sensitivities of identified bacteria varied significantly (p<0.05) to the synthetic antibiotics and aqueous extract of O. gratissimum. All the drugs tested showed greater antibacterial potentials in the control of most of the identified pathogen than the aqueous extract of the test plant. Streptomycine had the highest activity as it inhibited growth of all the bacteria. However, the aqueous extract had a great antibacterial potential in the control of Proteus mirabilis (inhibition zone, 14.50±0.71mm) and Micrococcus spp (inhibition zone, 17.50±0.71mm). The sensitivity of these bacteria to the extract of O. gratissimum is a pointer to its potential use in the formulation of new and more potent antibacterial drugs of natural origin in the production of C. gariepinus fingerlings.

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